Abstract
Molecular Beacon is a hairpin-shaped fluorescent probe,which can hybridize with great specificity target sequence that is complement to its loop sequence.The specificity of Molecular Beacon is as high as single base mismatch detection.Peptide Nucleic Acid(PNA)is an artificial synthesized analogue of nature occurring DNA,in which the DNA sugar-phosphate backbone has been replaced by a pseudo-peptide.Thus the hybridization of PNA to complement DNA strand is more specific and stable than that of DNA to DNA.PNA can stop polymerase extension reaction as well.In this paper,Molecular Beacons were employed to encode variables in satisfiability problem and complement PNA strands were added and allowed to hybridize with Molecular Beacon.The hybridized PNA strands on Molecular Beacon stopped polymerase extension reaction,causing Molecular Beacon corresponding to non-solution were digested by means of restriction endonuclease EcoRI.The remaining Molecular Beacons encoding solutions were read out via heating.The appealing characteristics of proposed method in this paper are:Reliable,no observation and record of midst solution,easy solution detection.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
