Molecular basis of the early events in plant–fungus interaction

Abstract

Fungal spores that land on aerial surfaces of plants first come into contact with plant cuticle. The cuticle is composed of an insoluble polyester called cutin, which is composed of hydroxy and hydroxyepoxy fatty acids and associated soluble waxes. The wax components can trigger differentiation of germinating fungal spores into infection structures. The penetration of the fungus into the plant requires enzymatic degradation of the polyester and the underlying carbohydrate barriers. The polyesterase, called cutinase, is induced by the contact with the plant surface. The small amount of cutinase carried by the spore generates a small amount of cutin monomers upon contact with the host, and the unique monomers trigger expression of the cutinase gene. This transcriptional level control can be demonstrated with isolated nuclei. Upon incubation of nuclei with cutin monomer and a soluble protein factor from the fungus, cutinase transcription is selectively activated. Structure activity relationships showed that the cutinase transcription activation required all of the structural elements of the cutin monomer. The cutinase transcript generated by the isolated nuclei was identical in size to the cutinase mRNA induced in the fungal cultures, which indicated usual initiation and termination. Fungal infection triggers defense reaction in plants. Plant peroxidases were implicated in the defense response of plants to fungal attack and stress. A highly anionic peroxidase involved in suberization of cell walls in tomato plants in response to fungal attack was cloned and sequenced. It was observed that in resistant lines of tomato the expression of this anionic peroxidase was induced 1 day earlier than in susceptible lines.Key words: cutinase, cutin monomers, pectate lyase, Fusarium solani pisi, anionic peroxidase.