Abstract
BackgroundCommon vetch (Vicia sativa L.) is a forage grain legume of high protein content and high nitrogen fixation, relevant in sustainable agriculture systems. Drought is the main limiting factor of this crop yield. Genetic resources collections are essential to provide genetic variability for breeding. The analysis of drought associated parameters has allowed us to identify drought tolerant and sensitive ecotypes in a vetch core collection.ResultsTo understand the mechanisms involved in drought response we analysed transcriptomic differences between tolerant and sensitive accessions. Polymorphic variants (SNPs and SSRs) in these differential expressed genes (DEGs) have also been analysed for the design of drought-associated markers. A total of 1332 transcripts were commonly deregulated in both genotypes under drought. To know the drought adaptive response, we also analysed DEGs between accessions. A total of 2646 transcripts are DEG between sensitive and tolerant ecotypes, in watered and drought conditions, including important genes involved in redox homeostasis, cell wall modifications and stress-response. The integration of this functional and genetic information will contribute to understand the molecular mechanisms of drought response and the adaptive mechanisms of drought tolerance in common vetch. The identification of polymorphic variants in these DEGs has also been screened for the design of drought-associated markers that could be used in future breeding program strategies.ConclusionsOur studies shed light for the first time in common vetch about the genes and pathways associated with drought tolerance. In addition, we identify over 100 potential drought associated polymorphism, as SNPs or SSRs, which are differently present in drought and tolerant genotypes. The use of these molecular markers for trait prediction would enable the development of genomic tools for future engineering strategies by screening of germplasm crop collections for traits related with crop drought resilience, adaptability or yield in vetch.
Highlights
Common vetch (Vicia sativa L.) is a forage grain legume of high protein content and high nitrogen fixation, relevant in sustainable agriculture systems
We found that water content decreased more quickly in accessions 284, 510 and 521, previously selected as drought tolerant candidates (Fig. 1b)
Our studies may help to understand the genes and molecular mechanisms associated with drought stress in common vetch
Summary
Common vetch (Vicia sativa L.) is a forage grain legume of high protein content and high nitrogen fixation, relevant in sustainable agriculture systems. Drought is the main limiting factor of this crop yield. The analysis of drought associated parameters has allowed us to identify drought tolerant and sensitive ecotypes in a vetch core collection. Drought causes large losses in the crop production in the arid and semiarid areas of the planet. Drought response includes a plethora of morphological, physiological, biochemical and molecular changes both at the whole plant and at cellular levels. Primary drought response entails reduction of relative water content and turgor alteration that activates stomatal closure, reduces transpiration and limits the photosynthesis rate. Important alterations are produced at radicular level where root architecture and physiology are affected to regulate homeostasis of water uptake to maintain osmotic pressure [1]. The more relevant aspects of plant responses to drought involve maintenance of osmotic and oxidative homeostasis. Drought promotes the accumulation of Reactive Oxygen Species (ROS) overcoming the antioxidant
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