Molecular based identification of Tem ß-lactamase and Tet a resistance gene in E. coli

Abstract

Escherichia coli is a universal bacterium causing infections in humans and animal and serves as a major pathogen of urinary tract infections (UTI) and Extraintestinal infection. The present study was conducted for current antibiotic resistance pattern of E. coli and molecular detection of resistance related gene in clinical isolates of E. coli. The study was a hospital based, prospective study which was done for a period of twelve months. This study was done by using the standard culture techniques for urine, pus, semen and sputum samples, Maximum number was from samples of urine 73 followed by pus 23 semen 2 and sputum 2. Hundred pathogenic E. coli isolates was further identified by standard microbiology techniques such as colony morphology, Gram staining and biochemical testing methods. Drug resistance was evaluated by disc diffusion method and relevant drug resistance gene detection done by Multiplex PCR. Out of 130 clinical samples total (n=100) isolates were identified as E. coli and their susceptibility patterns for different antibiotics were determined. Results showed that Gentamicin among aminoglycosides and Colistin sulfate among polymyxin were showed relatively less resistance in E. coli. Bacitracin, Ampicillin, trimethoprim, Erythromycin, Tetracycline, Ciprofloxacin, Amoxicillin and Piperacillin were found more resistant. Imepenum, Meropenum among ? lactam were most effective drug. PCR was employed to identify resistance causing gene. Among 100 pathogenic E. coli isolates 87% shown ampicillin resistance encoded by Tem B lactamase gene and 86 % shown tetracycline resistance causing by Tet A gene. Highest level of drug-resistance was observed against ampicillin and tetracycline (AMP-TET) among clinical isolates of pathogenic E. coli collected from hospitalized patients.