Abstract

Campylobacter jejuni and coli are collectively regarded as the most prevalent cause of bacterial foodborne illness worldwide. An emerging species, Campylobacter ureolyticus has recently been detected in patients with gastroenteritis, however, the source of this organism has, until now, remained unclear. Herein, we describe the molecular-based detection of this pathogen in bovine faeces (1/20) and unpasteurized milk (6/47) but not in poultry (chicken wings and caeca). This is, to the best of our knowledge, the first report of the presence of this potential gastrointestinal pathogen in an animal source, possibly suggesting a route for its transmission to humans.

Highlights

  • Correspondence Human campylobacterosis is generally regarded as a zoonosis with numerous reservoirs in the natural environment [1]

  • Ireland) identified C. ureolyticus in 23.8% of 349 previously genus-positive samples [6], making C. ureolyticus the second most common Campylobacter species detected in faecal samples of patients presenting with gastroenteritis in Southern Ireland

  • Amplicons were visualised by electrophoresis on agarose gels and the results were interpreted on the basis of presence/absence of a band for C. ureolyticus positive samples and an internal amplification control (IAC) amplification for C. ureolyticus negative samples

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Summary

Introduction

Correspondence Human campylobacterosis is generally regarded as a zoonosis with numerous reservoirs in the natural environment [1]. PCR following a multiplex PCR based detection system (EntericBio, Serosep Ltd. Limerick, Ireland) identified C. ureolyticus in 23.8% of 349 previously genus-positive samples [6], making C. ureolyticus the second most common Campylobacter species (after C. jejuni) detected in faecal samples of patients presenting with gastroenteritis in Southern Ireland. The presence of C. ureolyticus in each sample was investigated using PCR with a specific primer set targeting the hsp60 gene of C. ureolyticus

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Conclusion

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