Abstract
The Internal Transcribed Spacers (ITS) sequences of nuclear ribosomal DNA (nrDNA) are commonly used in plant molecular phylogenetics for the molecular based taxonomic identification and DNA barcoding because of shorter length and easy to amplify by using the universal primers, and further has discrimination ability to distinguish the taxon at lower taxonomic level. The present molecular phylogenetic analysis of ITS nrDNA sequences focuses to determine the taxonomic status of an unresolved medicinally important species Euphorbia schimperiana Scheele of the family Euphorbiaceae reported from Saudi Arabia. The combined length of the entire ITS region in E. schimperiana is 644 nucleotides. The study reveals that E. schimperiana shows a close proximity with the members of the subgenus Esula.
 Bangladesh J. Plant Taxon. 28(1): 125-130, 2021 (June)
Highlights
The Euphorbiaceae is a large family of flowering plants with about 300 genera and 7,500 species
The four main molecular phylogenetic studies of Euphorbia to date have revealed the overall phylogeny of the genus, with a major point of consensus being the recognition of four subgeneric clades: Rhizanthium, Esula, Euphorbia, and Chamaesyce (Steinmann and Porter, 2002; Bruyns et al, 2006; Park and Jansen, 2007; Zimmermann et al, 2010)
The length of the ITS1 region and GC contents in E. schimperiana was found consistent with some other earlier studies on the family Euphorbiaceae (Steinmann and Porter, 2002; Barres et al, 2011)
Summary
The Euphorbiaceae is a large family of flowering plants with about 300 genera and 7,500 species. The Internal Transcribed Spacers (ITS) of Nuclear Ribosomal DNA (nrDNA) in plants is being extensively used for phylogenetic studies, molecular discrimination of raw drug material and DNA barcoding (Ali et al, 2014). The DNA sequence of Euphorbia schimperiana has not been done before and is not available in the GenBank, the molecular evolutionary
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