Molecular assessment of microbial diversity on eggshell of chukar partridges (Alectoris chukar) under semi-captive conditions

Abstract

Due to human activity, climatic changes and habitat damage, the Chukar partridge (Alectoris chukar) is experiencing a population decrease. The goal of this work was to isolate and identify the inner eggshell microflora in order to learn more about their function in A. chukar embryo mortality and hatchability in semi-captive settings. During the egg-laying season, 204 eggs were gathered from the Balkasar Research Complex in Chakwal, Pakistan. These eggshells were divided into four categories after incubation: un-pricked with dead embryo, pricked with dead embryo, hatched, and unfertilized eggs. A total of 47 (23.03%) samples tested positive for gram-positive bacteria, which were then identified using 16S rRNA sequence analysis. Bacillus subtilis, Bacillus cereus, Bacillus amyloliquefaciens, Bacillus toyonensis, Bacillus thuringiensis and Staphylococcus sciuri were among the identified species. Hatched eggshells had the highest percentage of bacterial flora (36.17%), followed by un-pricked with dead embryo (23.40%) and pricked with death embryo (21.27%), and unfertilized eggshells had the lowest percentage (19.14%). As a result, the findings of this study revealed that microbial contamination of eggshells could be a cause of early embryonic stage degeneration and bird death.