Abstract
Aeromonas hydrophila is an important bacterial pathogen which causes the hemorrhagic septicemia in fishes, amphibians and humans. Genetic relationships of diverse isolates of A. hydrophila were recovered from fish and water sources. These isolates were investigated by flanked region of 16S and 23S Ribosomal DNA (rDNA) internal transcribed spacer (ITS). Here we analyzed polymorphism of PCR-amplified 16S-23S rDNA ITS and their revealed band pattern consisting of one to four DNA fragments. The fragment size ranged from 500 to 1000 bp. The DNA band patterns revealed a considerable genetic diversity in interaspecies. The 750 bp size of band was common in all isolates of A. hydrophila except one Isolates AH21. The tRNA-Glu sequences were identified from 750 bp size of ITS region that could be used as strain level potential genotypic markers.
Highlights
An epidemic of Aeromonas hydrophila infection with a high rate of mortality (95%) in turtles (Pseudemis scripta) has been reported in Italy [1]
The tRNA-Glu sequences were identified from 750 bp size of internal transcribed spacer (ITS) region that could be used as strain level potential genotypic markers
We have investigated the secondary structures of tRNA-Glu at different temperatures
Summary
An epidemic of Aeromonas hydrophila infection with a high rate of mortality (95%) in turtles (Pseudemis scripta) has been reported in Italy [1]. A. hydrophila is a significant bacterial pathogen of a wide variety of hosts, which is associated with hemorrhagic septicemia in fishes, reptiles and amphibians [7] In fishes, they cause well known diseases like hemorrhagic septicemia, fin as well as tail rot and result in the high mortality in commercial aquaculture system [8]. The pathogenicity assay has been performed in healthy fishes and found pathogenic bacteria such as A. hydrophila, A. bestiarum, A. salmonicida and A. veronii All these Aeromonas isolates were isolated from diseased trout except one which from carp fry [11]. Molecular characterization of bacteria is performed using PCR analysis of length polymorphism of the intergenic spacers lying between tRNA genes (tDNAPCR) [15]. The present study was to investigate the 16S-23S ITS PCR assay to discriminate and generate specific fingerprint at the strain level identification of diverse isolates of A. hydrophila recovered from diseased and apparently normal samples. We have investigated the secondary structures of tRNA-Glu at different temperatures
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