Molecular aspects on generalisations of splicing languages

Abstract

The mathematical modelling of DNA splicing systems is developed from the biological process of recombinant DNA where DNA molecules are cut and reassociated with the presence of a ligase and restriction enzymes. The molecules resulting from the splicing system generate a language which is known as a splicing language using formal language theory. In previous research, the splicing languages from different splicing systems have been generalised based on the sequences of restriction enzymes. In this research, the molecular aspects on the generalisations of splicing languages are discussed to validate the splicing languages through wet lab experiment. The initial string used in this model is taken from bacteriophage lambda. From the model, the predicted molecules resulting from the combination of the initial string and the chosen restriction enzymes are determined. The actual results from the experiment will be compared with the modelled results from the generalisations of splicing languages from DNA splicing system with palindromic and non-palindromic sequences of restriction enzymes with different crossings.