Abstract
ABSTRACT: Erythrina velutina Willd. (Fabaceae) is a Brazilian native tree with economic, ecological and social potential. The aim of this study was to evaluate isoenzyme activity and changes in DNA integrity during germination of E. velutina at different temperatures. The seeds were placed to germinate at 5, 15, 25, 35 and 45 °C, evaluating isoenzyme activity and degradation of the DNA during germination. Isoenzyme expression occurs differently for seed germination under different temperatures, with varied expression between seedlings and cotyledons. The esterase enzyme was more sensitive to express the response of the E. velutina germination seeds at different temperatures. DNA repair is more efficient during the germination of E. velutina seeds when submitted to a temperature of up to 25 °C, with damage to the genetic apparatus with an increase higher than this temperature.
Highlights
Erythrina velutina Willd. (Fabaceae) is a Brazilian native tree (Rodrigues et al, 2018)
The seedling did not express this isoenzyme at any of the evaluated temperatures, to the work of Tunes et al (2011), in which the alcohol dehydrogenase (ADH) activity was quite pronounced in the seeds, whereas it was practically null in the seedlings
This enzyme is stored in the seed during the maturation process, and remains stable until germination begins, and its metabolism is reactivated in phases I and II of imbibition. Their levels decrease drastically after imbibition (Weitbrecht et al, 2011), indicating the predominance of the aerobic process of energy generation. This is consistent with the low intensity of ADH expression observed in the electrophoretic profile from E. velutina cotyledons after 20 days of seed imbibition
Summary
Erythrina velutina Willd. (Fabaceae) is a Brazilian native tree (Rodrigues et al, 2018). The leaf extracts contain phenols, tannins, alkaloids, flavonoids, sterols, triterpenoids, catechins, saponins and xanthones (Palumbo et al, 2016, Rodrigues et al, 2017). Ecological and economic importance of E. velutina to the Northeast region of Brazil (Caatinga and Cerrado phytogeographic domains), there is still little information on this species, especially related to the physiological and biochemical aspects during germination. In this process, unfavorable environmental conditions have been affecting the initial establishment and development in the field. The optimal germination temperature is that for which the seed expresses its maximum germination performance, while the minimum and the maximum are characterized by lower germination, at which germination does not occur at below or above it (Bewley et al, 2013)
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