Molecular and serological detection of Toxoplasma gondii infection in mammals in the Taipei Zoo.

Abstract

Toxoplasmosis caused by Toxoplasma gondii affects both conservation and public health efforts. In the Taipei Zoo, toxoplasmosis was diagnosed in ring-tailed lemurs and a meerkat in 2019 while a freeze-thaw meat strategy had been applied to carnivores before the event. To investigate the possible risk factors associated with T.gondii infection in the Taipei Zoo, 179 veterinary visiting mammals from 2019-2021 and six stray cats were included to detect anti-T.gondii IgG and IgM in their serum via ELISA, and T. gondii in their faeces and blood via PCR. Although the overall T.gondii IgG seroprevalence was 33.5% and PCR positivity was 16.2% in the zoo mammals, the correlation between T.gondii PCR and systemic IgG results was low. An omnivorous diet (adjusted OR=0.4; 95% CI: 0.2-1.0), a herbivorous diet (adjusted OR=3.2; 95% CI: 1.1-9.6), and animals in the Conservation Area where stray cats appeared (adjusted OR=18.3; 95% CI: 3.9-85.9) were independent risk factors for T.gondii infection. The low T.gondii-specific IgM positivity (0.6%) suggests that most animals did not have acute T.gondii infection. In conclusion, our findings indirectly support that feeding frozen meat to carnivores, cleaning fresh food, and restricting access to stray cats to prevent faecal contaminants could prevent animals from T.gondii exposure.