Molecular and serological detection of avian infectious bronchitis virus in vaccinated and non- vaccinated chickens in Wasit province/Iraq

Abstract

The present study was conducted to evaluate the efficacy of IBV vaccine by detecting presence of the infectious bronchitis virus (IBV) by enzyme linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR) techniques. 180 serum sample were collected from chicken farms including (154) samples were collected from clinically infected vaccinated broiler farm and (26) serum samples were collected from clinically infected non-vaccinated broiler farm. Samples submitted for ELISA assay, The results were as follow 90(98%)positive and 64 sample negative in ELISA test from vaccinated farms, ELISA results showed also 2(7.6%) sample positive and 24(92.3%) negative from non-vaccinated farms. Eighty sample(60 sample were positive and 20 were negative with ELISA test) were submitted for the presence of IBV by RT-PCR technique. Virus was detected in 37(45.25 %), while 43( 66.25%) did not have detectable IBV according to RT-PCR results.