Molecular and pathological identification of maize genotypes having Wsm gene governing resistance against MDMV and MCDV

Lutful Hassan,Md Ashraful Haque,Shanjida Rahman,Upama Mondal

doi:10.3329/jbau.v16i2.37962

Abstract

Maize is seriously affected by different viruses like Maize Dwarf Mosaic Virus (MDMV) and Maize Chlorotic Dwarf Virus (MCDV) throughout the world. In Bangladesh, no genotype has been identified yet as a source of resistant gene against MDMV and MCDV. The study was carried out with the objective to screen nine maize genotypes carrying Wsm gene using three sets of Single Sequence Repeat (SSR) marker. Maize plants were inoculated with viruses. Symptoms were scored at 7, 10 and 14 dpi (days post inoculation) to calculate infection percentage and Area Under Disease Progress Curve (AUDPC). The molecular result indicated that BHM-7, BHM-5, V-92, Uttaran and Duranta carried Wsm gene, but according to pathological test, functional resistance was observed for only BHM-7, V-92 and Uttaran on the basis of infection percentage and AUDPC score. BHM-7 (BARI Hybrid Maize 7) was noticed as the best one for showing resistance against MDMV and carrying Wsm gene. No genotype was found to govern resistance against MCDV.J. Bangladesh Agril. Univ. 16(2): 208-214, August 2018

Highlights

Maize (Zea mays) is an important crop throughout the world including Bangladesh which occupies a large portion of world economy (Gong et al, 2015; Ranum et al, 2014; Ahmed, 2013; Roper, 2013; Moniruzzaman et al, 2009; Paliwal et al, 2000; Dowswell et al, 1996)
Maize dwarf mosaic disease is one of them which is caused by a series of viruses including Maize dwarf mosaic virus (MDMV), Maize chlorotic dwarf virus (MCDV), Sugarcane mosaic virus (SCMV), Johnsongrass mosaic virus (JGMV), Sorghum mosaic virus (SrMV), Zea mosaic virus (ZeMV) and Pennisetum mosaic virus (PenMV) (Zambrano et al, 2014, Stewart et al, 2012; Stewart et al, 2013)
All of them except MCDV are classified as the “sugarcane mosaic subgroup” of the virus genus Potyvirus, under the family Potyviridae (Balarabe et al, 2014, Haider et al, 2011, Mohammadi and Hajieghrari 2009, Shukla et al, 1992)

Summary

Introduction

Maize (Zea mays) is an important crop throughout the world including Bangladesh which occupies a large portion of world economy (Gong et al, 2015; Ranum et al, 2014; Ahmed, 2013; Roper, 2013; Moniruzzaman et al, 2009; Paliwal et al, 2000; Dowswell et al, 1996). Maize dwarf mosaic disease is one of them which is caused by a series of viruses including Maize dwarf mosaic virus (MDMV), Maize chlorotic dwarf virus (MCDV), Sugarcane mosaic virus (SCMV), Johnsongrass mosaic virus (JGMV), Sorghum mosaic virus (SrMV), Zea mosaic virus (ZeMV) and Pennisetum mosaic virus (PenMV) (Zambrano et al, 2014, Stewart et al, 2012; Stewart et al, 2013). All of them except MCDV are classified as the “sugarcane mosaic subgroup” of the virus genus Potyvirus, under the family Potyviridae (Balarabe et al, 2014, Haider et al, 2011, Mohammadi and Hajieghrari 2009, Shukla et al, 1992). Potyviruses are economically important groups of plant viruses that pose a dangerous threat to crops around the world (Dujovny et al, 2000; Moriones and Luis-Arteaga, 2000; Inoue-Nagata et al, 2002; Larsen et al, 2003)

Methods

Discussion

Conclusion