Abstract
Fungi of Guignardia genus are commonly isolated from different plant species and most of the time, they are characterized as endophytes. However, some species of this genus, like G. citricarpa and G. psidii are known as causal agents of serious diseases that affect important crops such as Citrus Black Spot and guava fruit rot, respectively. They are also responsible for diseases that cause foliar spots in different fruit species and also in other crops, but cause minor damages. Despite evidences that G. mangiferae colonizes different plant species, there are few studies about its genetic diversity associated with different hosts. This work has the objective to characterize Guignardia isolates obtained from different hosts and tissues by RAPD, fAFLP and DNA sequence of ITS1-5.8S-ITS2 region, as well as to develop pathogenicity tests through cross inoculation in citrus and guava fruits. It was observed that molecular markers were able to discriminate isolates of different Guignardia species. Pathogenicity tests showed that G. citricarpa caused CBS symptoms on citrus fruits, but it did not produce any symptoms in guava fruits. G. mangiferae isolates were able to cause rot symptoms on guava fruits, but they have not produced any symptoms on citrus fruits. Guignardia isolates obtained from mango leaves that have not been classified in species have not presented any symptoms in citrus and guava fruits. Although G. mangiferae is commonly isolated asymptomatically in different plants, this work supports the evidence that this species has a latent pathogen behavior, at least for guava plants.
Highlights
The Guignardia Genus (Kingdom Fungi, Phylum Ascomycota, Class Dothideomycetes, Order Botryosphaeriales, Family Botryosphaeriaceae) encompasses around 330 known species, many of them with unknown anamorphic phase [1]
Black Spot (CBS) G. citricarpa [2], and G. psidii species causing fruit rot in guava
Studies using molecular techniques suggested that fungi isolates identified as G. psidii could be G. mangiferae, or could be conspecific to this cosmopolitan species [4]
Summary
The Guignardia Genus (Kingdom Fungi, Phylum Ascomycota, Class Dothideomycetes, Order Botryosphaeriales, Family Botryosphaeriaceae) encompasses around 330 known species, many of them with unknown anamorphic phase [1]. Many species considered plant endophytic fungi are classified in this family and genus, among them, the G. mangiferae species, the causal agents of Citrus. Black Spot (CBS) G. citricarpa [2], and G. psidii species causing fruit rot in guava. The G. psidii species is considered responsible for fruit rot disease in different plants, mainly in postharvest conditions. This fungus species is responsible for great losses in guava fruits in Brazil [3]. Studies using molecular techniques suggested that fungi isolates identified as G. psidii could be G. mangiferae, or could be conspecific to this cosmopolitan species [4]. It is very common that organisms belonging to the same species, when obtained from dif-
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
