Abstract
Alfalfa mosaic virus (AMV) is one of the most important viruses that cause significant losses to alfalfa worldwide. A total of 1066 symptomatic alfalfa, 143 weeds and other secondary hosts were collected and assayed for AMV infection. The virus was detected in 360 samples (33.8% incidence) of alfalfa by plate-trapped antigen (PTA)-ELISA. Among the weed species and the other plants tested, potato, pepper and seven weed species were infected with AMV. The coat protein (CP) gene sequences from 59 Iranian isolates were PCR amplified, cloned, sequenced, and compared with the sequences available in GenBank. Sequence analysis of the amplified regions showed that 55 of the Iranian CP sequences share >98% pairwise identity with each other. The remaining four share between 89 and 96% identity. Our host studies indicated that the Iranian AMV isolates were classified into two groups. Only four Iranian isolates were clustered in the symptom-group B and caused relatively milder symptoms compared to those found in symptom-group A.
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