Abstract
A field experiment was carried out at the Department of Horticulture and Landscape Gardening, College of Agricultural Engineering Sciences, University of Baghdad .during 2016. to investigate the genetic diversity for six genotype of snake melon Cucumis melo var. flexuosus (Qutha ), cultivated in Iraq. These selected genotypes were collected from Iraq's Governorates (Baghdad, Sulaymaniyah, Mosul, Salahadeein, and Diyala )in addition to commercial genotype and designated as A,B,C,D,E, and F, respectively. The treatments were arranged in randomized complete block design (RCBD) with three replications Genetic diversity among the six selected genotype was studied utilizing the random amplification of polymorphic DNA (RAPD) markers and linked with fruits morphological traits. The results showed that the highest genetic diversity (0.285) observed between the genotype (A and B ) and between (A and D) was (0.235) ,while the genotype (C and D) had the lowest genetic distances which was (0.031), as well as between( E and F) was (0.059). Also the results revealed that Genetic distances depend on the variance phenotypic of fruits recorded about (1 ) as highest number among the genotype (A and D) , (B and D ), (C and D ), (D and E ), and (D and F ), while the lowest was about ( 0.091 ) for (A and C.)Genotype
Highlights
A field experiment was carried out at the Department of Horticulture and Landscape Gardening, College of Agricultural Engineering Sciences, University of Baghdad .during 2016. to investigate the genetic diversity for six genotype of snake melon Cucumis melo var. flexuosus (Qutha ), cultivated in Iraq
Snake melon Cucumis melo var. flexuosus related to the Cucurbitaceae family
Showed that 50 snake melon accessions collected from Palestine (West Bank), 17 of the phenotypic characters were polymorphic. These accessions related to four important genotype of Cucumis melo var. flexuosus
Summary
Snake melon Cucumis melo var. flexuosus related to the Cucurbitaceae family. It is a morphologically diverse crop with very polymorphic fruit types (6,8 ) It is commonly known by the name qutha in Iraq, where many genotype can be found. During the early history of plant breeding, the markers were mainly used visible morphological traits such as leaf shape, flower color, pubescence color, pod color, seed color, seed shape, helium color, awn type and length, fruit shape, rind (exocarp) color and stripes, flesh color, stem length, etc These morphological markers generally represent genetic polymorphisms which are identified and manipulated.Randomly. Showed that 50 snake melon accessions collected from Palestine (West Bank), 17 of the phenotypic characters were polymorphic These accessions related to four important genotype of Cucumis melo var. Molecular diversity A second experiment was included molecular fingerprinting of snake melon accessions which based on the Randomly Amplified Polymorphic of DNA (RAPD), investigate in Biotechnology Center, University of ALNahrain, Leaves samples The experiment was carried out in pots under greenhouse condition, where 5 seeds were sown in each pot. Leaves sample were taken after 4 weeks from the sowing date, which will be used for the molecular electrophoresis with red safe nucleic acid stain assessment
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