Molecular and Histopathological Study on the Ameliorative Impacts of Petroselinum Crispum and Apium Graveolens against Experimental Hyperuricemia

Mohamed Mohamed Soliman,Wafaa Abdou Mohamed,Fayez Althobaiti,Mohamed Abdo Nassan,Adil Aldhahrani

doi:10.1038/s41598-020-66205-4

Mohamed Mohamed Soliman, Wafaa Abdou Mohamed + Show 3 more

Open Access

https://doi.org/10.1038/s41598-020-66205-4

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Abstract

Hyperuricemia is an abnormal metabolic condition characterized by an increase in uric acid levels in the blood. It is the cause of gout, manifested by inflammatory arthritis, pain and disability. This study examined the possible ameliorative impacts of parsley (PAR) and celery (CEL) as hypouricemic agents at biochemical, molecular and cellular levels. PAR and CEL alone or in combination were orally administered to hyperuricemic (HU) mice and control mice for 10 consecutive days. Serum levels of uric acid and blood urea nitrogen (BUN), xanthine oxidase activity, antioxidants, inflammatory (IL-1β and TNF-α) and anti-inflammatory cytokines (IL-10) were measured. mRNA expression of urate transporters and uric acid excretion genes in renal tissues were examined using qRT-PCR (quantitative real time PCR). Normal histology and immunoreactivity of transforming growth factor-beta 1 (TGF-β1) in kidneys was examined. Administration of PAR and CEL significantly reduced serum BUN and uric acids in HU mice, ameliorated changes in malondialdehyde, catalase, and reduced glutathione, glutathione peroxidase (GPX), IL-1β, TNF-α and IL-10 in hyperuricemic mice. Both effectively normalized the alterations in mURAT-1, mGLUT-9, mOAT-1 and mOAT-3 expression, as well as changes in TGF-β1 immunoreactivity. Interestingly, combined administration of PAR and CEL mitigated all examined measurements synergistically, and improved renal dysfunction in the hyperuricemic mice. The study concluded that PAR and CEL can potentially reduce damaging cellular, molecular and biochemical effects of hyperuricemia both individually and in combination.

Highlights

The use of organic drugs and therapies is cost-effective[12]
This study aimed to investigate the anti-hyperuricemic activity of parsley and celery at cellular and molecular levels on experimental hyperuricemia induced by oxonate in mice
The kits for blood urea nitrogen (BUN), uric acid, creatinine, GOT and GPT were from EGY-CHEM for lab technology (Badr City, Egypt)

Summary

Introduction

The use of organic drugs and therapies is cost-effective[12]. The positive and promising effects of medicinal herbs on renal diseases, infertility, liver disorders and diabetes are clearly established and are accepted by patients and clinicians as a safe medication for these disorders[13,14,15,16,17]. Plants of medical importance contain flavonoids and www.nature.com/scientificreports other phenolic compounds that have strong antioxidant effects, and have been investigated in many studies[15,16,17]. Parsley is the most well-known antioxidant-rich herb that reduces inflammation, especially in the joints[20]. Parsley prevents cancer cells from differentiation and growth[23], and is a safe, natural remedy to reduce glucose levels in diabetic individuals[24]. Leaves, and essential oil extracts are widely used in medicine. Flavonoids, selinene, and frocoumarin glycosides are the main compounds present in celery, making it one of the most commonly used plants in traditional medicine[25]. This study aimed to investigate the anti-hyperuricemic activity of parsley and celery at cellular and molecular levels on experimental hyperuricemia induced by oxonate in mice

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