Abstract
Genetically-encoded biosensors based on fluorescence proteins (FPs) and fluorescence resonance energy transfer (FRET) have enabled the specific targeting and visualization of signaling events in live cells with high spatiotem... | Find, read and cite all the research you need on Tech Science Press
Highlights
Genetically-encoded biosensors based on fluorescence proteins (FPs) and fluorescence resonance energy transfer (FRET) have enabled the specific targeting and visualization of signaling events in live cells with high spatiotemporal resolutions
We have a developed a general highthroughput screening (HTS) method based on directed evolution to develop sensitive and specific FRET biosensors
We have first applied a yeast library and screened for a mutated binding domain for phosphorylated peptide sequence. When this mutated binding domain and the peptide sequence are connected by a linker and concatenated in between a pair of FRET FPs, a drastic increase in sensitivity can be achieved
Summary
Genetically-encoded biosensors based on fluorescence proteins (FPs) and fluorescence resonance energy transfer (FRET) have enabled the specific targeting and visualization of signaling events in live cells with high spatiotemporal resolutions. Single-molecule FRET biosensors have been successfully developed to monitor the activity of variety of signaling molecules, including tyrosine/serine/threonine kinases.
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