Molecular and Biological Characterization of Spodoptera frugiperda Multiple Nucleopolyhedrovirus Field Isolate and Genotypes from China.

Chengfeng Lei,Jia Hu,Jia Wang,Xiulian Sun,Jian Yang

doi:10.3390/insects11110777

Abstract

Simple SummaryThe fall armyworm is a notorious lepidopteran pest that consumes many economically important crops. Its recent invasion into China threatens crops in over 19 provinces. This species is susceptible to its homologous nucleopolyhedrovirus (Spodoptera frugiperda multiple nucleopolyhedrovirus, or SfMNPV). Selection of indigenous isolates that are adaptable in each geographical region is important for developing a virus-based pesticide. In this study, an SfMNPV field isolate was obtained from a natural population of the fall armyworm in Hubei, China. Two genotypes were cloned from the field isolate, and one genotype, SfHub-A, which had similar activity to the field isolate and produced significantly more progeny viruses, was considered to be a suitable strain for the commercial production of SfMNPV. This information will be valuable for developing a virus-based pesticide against fall armyworm in China.The fall armyworm, Spodoptera frugiperda, is a new invading pest in China. The baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) is a pathogenic agent of the fall armyworm and a potential agent for its control in integrated pest management strategies. In this work, we analyze the molecular and biological characteristics of an SfMNPV isolate collected from maize in China (SfMNPV-Hub). Two genotypes were further isolated from SfMNPV-Hub by an in vivo cloning method. The PstI profile of one genotype (SfHub-A) was similar to genotype A of the SfMNPV Colombian isolate, and the other (SfHub-E) was similar to genotype E of the Colombian isolate. The bioactivity of SfHub-A against second-instar S. frugiperda larvae was not significantly different from that of SfMNPV-Hub, whereas SfHub-E was 2.7–5.5 fold less potent than SfMNPV-Hub. The speed of kill of SfHub-E was quicker than SfMNPV-Hub, while SfHub-A acted slower than SfMNPV-Hub. Occlusion body (OB) production of SfHub-A in an S. frugiperda cadaver was significantly higher than that of SfMNPV-Hub, while SfHub-E yielded far fewer occlusion bodies (OBs) in the host larvae. These results provide basic information for developing a virus-based pesticide against the invading pest S. frugiperda.

Highlights

The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is a highly fecund, adaptable, and polyphagous lepidopteran that consumes many plant species, including economically importantInsects 2020, 11, 777; doi:10.3390/insects11110777 www.mdpi.com/journal/insectsInsects 2020, 11, 777 crops, such as maize, soybeans, and sorghum [1]
The larvae showed typical of baculovirus infection, and numerous Occlusion body (OB) could be collected from the cadavers
Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) has been developed as a commercial insecticide [32], and the selection of indigenous isolates is important for developing a virus-based pesticide against S. frugiperda [20]

Summary

Introduction

Insects 2020, 11, 777 crops, such as maize, soybeans, and sorghum [1]. A serious pest in most of North and South America, it was recently introduced into Africa, Asia, and Oceania, where it threatens crops in over 110 countries [2]. Baculoviruses are important pathogens for a wide range of insect pests [4,5]. Field observations indicate that S. frugiperda is susceptible to a homologous Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) that causes natural epizootics of the disease [6]. This has motivated studies focused on the development of SfMNPV as a biological insecticide [7,8,9,10].

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