Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus.

Fránová Fránová,Koloniuk Koloniuk,Přibylová Přibylová

doi:10.3390/v11110982

Fránová Fránová, Koloniuk Koloniuk + Show 1 more

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https://doi.org/10.3390/v11110982

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Abstract

Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus Fragaria thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects F. ananassa and F. vesca plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single F. ananassa host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via Aphis fabae and A. ruborum, further mechanically transmitted to Nicotiana occidentalis 37B and sub-inoculated to N. benthamiana, N. benthamiana DCL2/4i, N. occidentalis 37B, and Physalis floridana plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed F. vesca clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus Cytorhabdovirus. Acyrthosiphon malvae, A. fabae, and A. ruborum were shown to transmit StrV-1 under experimental conditions.

Highlights

Strawberry, as a very popular commercial and garden fruit and nutritionally important fruit, has long been cultivated worldwide [1]
A recent study showed that the number and weight of fruits from strawberry plants with an asymptomatic infection with only strawberry mild yellow edge virus (SMYEV, family: Alphaflexiviridae, genus: Potexvirus) was reduced by 28% to 63% compared with that of healthy plants, depending on the parameter measured and the production cycle [12,13]
For high-throughput sequencing (HTS), three strawberry plants showing symptoms resembling those of viral infection were selected: (i) F. ananassa Duch. cv

Summary

Introduction

Strawberry, as a very popular commercial and garden fruit and nutritionally important fruit, has long been cultivated worldwide [1]. Viruses in strawberry plants are found at low concentrations and in mixed infections, and commonly induce non-specific plant symptoms [3,4]. Multiple viral infections in plants can lead to yield loss and plant decline [2,3,5,6,7,8,9,10,11]. A recent study showed that the number and weight of fruits from strawberry plants with an asymptomatic infection with only strawberry mild yellow edge virus (SMYEV, family: Alphaflexiviridae, genus: Potexvirus) was reduced by 28% to 63% compared with that of healthy plants, depending on the parameter measured and the production cycle [12,13]

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