Abstract
In recent years, the accumulated molecular data of Turnip mosaic virus (TuMV) isolates from various hosts originating from different parts of the world considerably helped to understand the genetic complexity and evolutionary history of the virus. In this work, four complete TuMV genomes (HC9, PK1, MS04, MS15) were characterised from naturally infected cultivated and wild-growing Papaver spp., hosts from which only very scarce data were available previously. Phylogenetic analyses showed the affiliation of Slovak Papaver isolates to the world-B and basal-B groups. The PK1 isolate showed a novel intra-lineage recombination pattern, further confirming the important role of recombination in the shaping of TuMV genetic diversity. Biological assays indicated that the intensity of symptoms in experimentally inoculated oilseed poppy are correlated to TuMV accumulation level in leaves. This is the first report of TuMV in poppy plants in Slovakia.
Highlights
The Papaver genus in the Papaveraceae family comprises up to 100 plant species distributed in various areas of the world
In an effort to assess the presence of viral pathogens naturally infecting poppy, total RNAs isolated from leaves of an oilseed poppy plant showing virus-like symptoms were subjected to high throughput sequencing (HTS)
The analysis of the sequence data obtained from this sample (HC9, ca. 2.1 millions of reads of mean length 89.7 nucleotides) enabled us to obtain a large contig of ca. 9 kb, corresponding to Turnip mosaic virus (TuMV)
Summary
The Papaver genus in the Papaveraceae family comprises up to 100 plant species distributed in various areas of the world. Common poppy is a red-flowering agricultural weed abundantly spread in Europe [4] Few viruses infecting these Papaver spp. have been reported to date [5]. A new umbravirus, Opium poppy mosaic virus, was identified as causing leaf mosaic and mottling in opium poppy in New Zealand [7] and Tomato leaf curl New Delhi virus was detected from poppies showing curling of leaves in India [8]. Despite these reports, knowledge of viral pathogens naturally infecting poppy remains limited. Biological assays indicated that intensity of symptoms induced in experimentally inoculated oilseed poppy were correlated with the virus accumulation levels in leaves
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