Molecular and Biochemical Effect of Neem Extract On Experimental Diabetes

Abstract

Return to nature is now adays target of view so the aim of this study to evaluate aqueous extract effects of Neem leaf on streptozotocin (STZ) experimental diabetic rats. Sixty male rats were divided equally into 4 groups. first group used as healthy normal control, second group injected with STZ (50 mg/kg.B.W) and act as control diabetic, third group injected I/P with STZ for induction of diabetes and treated with Neem leaf extract (200 mg/kg.B.W orally / day) for 30 days and the fourth group administrated with Neem leaf extract for 15 days before induction of diabetes, then during and after injection of STZ .for 30 days Blood samples were collected at 15 & 30 days from onset of treatment. Serum was directed for estimation of glucose, lipid profile, insulin hormone, insulin receptor, HMG-coA reductase, cortisol and total IgE . Hepatic tissue subjected to PCR for stimulation insulin receptors. The recorded results revealed significant increase in glucose, cholesterol, TG, insulin and HMGcoA reductase in diabetic rats when compared to control .The treatment with Neem extract resulted in significant decrease in all these parameters whereas serum cortisol has non significant change in all groups, while HDL showed non change in diabetic. In conclusion Neem act as potent antidiabetic, antihyperlipidemic and has a good molecular activity on insulin receptor gene expression by PCR