Abstract
The structure of the wild-type waxy (wx+) locus was determined by sequence analysis of both a genomic and an almost full-size cDNA clone. The coding region comprises 3,718 bp and is composed of 14 exons and 13 small introns. The exons and the promoter region are G/C rich (60%–80%). All three waxy transcripts analysed so far reveal different polyadenylation sites and corresponding polyadenylation signals. The smallest of these mRNAs has a size of 2,263 nucleotides. Northern blot analysis suggests that the tissue-specific expression of the locus is due to transcriptional control. The insertion sites of all transposable element induced waxy mutations analysed have been mapped precisely within the locus. N-terminal sequencing of the mature wx+ protein leads to the identification of a maize amyloplast-specific transit peptide of 72 aminoacid residues.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.