Molecular Analysis of the Antiseptic Resistance Genes among Multidrug-Resistant Pseudomonas aeruginosa Isolated from Burn Patients

Abstract

Abstract Background: Pseudomonas aeruginosa is known to be susceptible to a variety of antibiotics and disinfectants, and this resistance has become a major problem, particularly among hospitalized patients. Objectives: The present study aims to determine the association between class 1 integrons (intI1) and genes responsible for antiseptic resistance (fabV, qacΔE1, and qacE) among multidrug-resistant (MDR) P. aeruginosa from burn patients. Materials and Methods: Sixty-four isolates from burns were identified using both standard approaches and the VITEK 2 system. Disc diffusion antimicrobial susceptibility testing was performed. Polymerase chain reaction (PCR) was utilized to identify class 1 integrons and antiseptic resistance genes using specific primers for intI1, fabV, qacE1, and qacE. Results: 64 MDR P. aeruginosa isolates were obtained from burn patients during the research period. The antibiotic susceptibility of all 64 P. aeruginosa isolates was examined. MDR resistance to at least three antibiotic classes were found in all of the isolates. The PCR results revealed that 100% of the isolates tested positive for the class 1 integron gene. According to the current findings, all isolates possessed the qacΔE1 and fabV genes. While the qacE results were being processed, 87.50% of the isolates tested positive. There was no correlation between the presence of antiseptic resistance genes and antibiotic resistance (P ˃ 0.05). Conclusion: our data suggest that P. aeruginosa isolates have profound antibiotic resistance and an antiseptic resistance gene. The integron was mostly found in all isolates, indicating that the Antiseptic resistance gene corresponds to the integron class 1.