Abstract
The present report describes the cloning, characterization and expression analysis of squalene epoxidase (SE) gene from C. borivilianum (Sant. and Fernand.). SE catalyzes the stereospecific epoxidation of squalene to 2, 3-oxidosqualene and is one of the rate limiting enzymes in the biosynthesis of saponins. A full length cDNA of CbSE contained 1,893 bp with an open reading frame of 1,599 bp, encoding a protein of 533 amino acids with conserved FAD, NAD(P) and squalene epoxidase domains. Phylogenetic analysis and 3D structure prediction using various programs showed CbSE structure to be similar to SE from other species. Open Reading Frame of SE was expressed in E. coli M15 cells, induced with 1 mM IPTG at 37 °C. Comparative expression analysis by semi-quantitative PCR demonstrated the high transcript levels of CbSE in leaf tissues as compared to root tissues.
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