Abstract
Present study is aimed to identify and analyse the genetic character of N. virescens and also its phylogeny to related species based on mt COI or DNA barcoding. The steps of this genetic analysis are leafhopper sample catching in rice field, DNA extraction, DNA amplification, product purification and DNA sequencing. Primer COI AB 976528 has been used in mtCOI-DNA amplification step. The research result showed that mtCOI-DNA fragment of N. virecens has length 677–681 bp. This mtCOI-DNA gene sequence was dominated by A and T bases with concentration 69.59%, whereas CG content was 30.39%. The concentration average of T (U), C, A and G nucleotides in the mtCOI gene sequence were 37.83, 15.96, 31.76 and 14.43%, respectively. Based on molecular phylogeny analysis, green leafhopper N. virescens Samosir populations was similar (98.60%) into green leafhopper N. virescens populations originated from Bangladesh. Identification of N. virescens based on this mtCOI-DNA gene sequence confirmed the identification result based on its morphological characters.
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