Abstract
Complete DNA sequences of the small subunit ribosomal RNA (SSUrRNA) gene and partial sequences of three other loci were obtained from three variant-type and three classic-type Plasmodium ovale isolates from Southeast Asia and compared with GenBank-available data. Three different SSUrRNA sequences (Pov 1-3) were found in each variant-type isolate, and two different SSUrRNA sequences (Poc 1-2) in each classic-type isolate. Pov 1-3 were closer to sequences previously found in the Cameroon and MAL/MAI isolates, whereas Poc 1-2 were closer to sequences previously found in two clones of the Nigerian I/CDC strain. The 3' half of Pov 1-3 was identical to the partial sequence of the SSUrRNA gene from the London School (LS) strain. Results support grouping P. ovale into two groups, the classic type (including the Nigerian I/CDC strain) and the variant type (Cameroon, MAL/MAI, and LS isolates).
Highlights
Complete DNA sequences of the small subunit ribosomal RNA (SSUrRNA) gene and partial sequences of three other loci were obtained from three variant-type and three classic-type Plasmodium ovale isolates from Southeast Asia and compared with GenBank-available data
Sequence Analysis of the Full SSUrRNA Gene Three different sequences were obtained for the SSUrRNA gene from each variant-type isolate, while two different sequences were detected from each classic-type isolate (Figure 1)
The presence of London School (LS)-type or variant-type P. ovale was confirmed in Vietnam [4] and Africa [7]; all varianttype isolates shared the same mutations at the block 9 in the SSUrRNA gene
Summary
Complete DNA sequences of the small subunit ribosomal RNA (SSUrRNA) gene and partial sequences of three other loci were obtained from three variant-type and three classic-type Plasmodium ovale isolates from Southeast Asia and compared with GenBank-available data. During our previous molecular studies of P. ovale in southern Vietnam [4], we found two field isolates whose partial sequences at the block 9 region [5] of the small subunit ribosomal RNA (SSUrRNA) genes had a deletion of 2 nt (G-G) and a substitution of 1 nt (C to T), when compared with the classic type, the Nigerian I/CDC strain [6] These polymorphisms had practical implications, since they occurred in the target of a diagnostic oligonucleotide probe used by the commercially available microtiter-plate hybridization (MPH) method for malaria diagnosis [4]. Our analyses of both nuclear and mitochondrial genes provide further support to the division of P. ovale into at least two types
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