Molecular analysis of mutant of potato (Solanum tuberosum L.) cultivar Atlantic

Abstract

Gamma irradiation has been applied to potato (Solanum tuberosumL.) cultivar Atlantic and IPB CP1 cultivar is one of mutant clones had been obtained. RAPD was applied as molecular marker to analyze the difference between the potato cv IPB CP1 and cultivar Atlantic. A total of 24 primers composed of single random primers and combination of two random primers were used to amplify DNA fragments from the genomic DNA of potato cultivar IPB CP1 and Atlantic. These primers are considered as loci and the amplicon generated by this primer is considered as allele. The number of allele indicated by the number of amplified DNA fragment ranged from 3 to 16 alleles depending on the locus represented by the primer. The combination of two random primers produced the highest polymorphic fragments. The difference between potato cultivar IPB CP1 and Atlantic is determined by 4 loci among 24 loci of RAPD, as OPA-02, OPA-02 + OPA-03, OPA-02 + OPA-07, and OPA- 01 + OPA-13. Locus OPA-02 + OPA-03 contains the highest genetic difference with 2 polymorphic fragments (22.22 %). This research had succesfully detected molecular difference between potato cv Atlantic and IPB CP1 as its mutant using RAPD marker.