Abstract
The understanding of the molecular and biochemical characteristics of the human leukocyte antigen-G (HLA-G) is important because of the diverse influence of this antigen’s polymorphisms on the course of a pregnancy. The aim of our study was to assess how the variation of the HLA-G allele and the HLA-G 14-bp ins/del polymorphism influence predisposition to a complicated pregnancy. The clinical material consisted of parental pairs with complicated pregnancies (210 women; 190 men). The control group included parental pairs without complications during pregnancy (89 women; 86 men). The study involved isolation of genome DNA from peripheral blood leukocytes, sequencing, and analysis of the 14-bp ins/del polymorphism in the 3′-untranslated region (3′-UTR) of the HLA-G gene based on polymerase chain reaction (PCR). The most common HLA-G allele in the group of women with complicated pregnancies was the HLA-G 10101 allele. There were no statistically significant differences in the frequencies of the 14-bp ins/del polymorphism in the 3′UTR of the HLA-G gene between the groups. Our results suggest that the risk of complications in pregnancy is influenced by the HLA-G 10101, HLA-G 10108, and HLA-G 10106 alleles and is not influenced by the 14-bp ins/del polymorphism in the 3′UTR of the HLA-G gene.
Highlights
During embryo implantation and pregnancy, the maternal immune system comes into close contact with fetal trophoblast cells
The aim of this study was to assess how the variation of the human leukocyte antigen-G (HLA-G) allele and the HLA-G 14-bp ins/del polymorphism in parental pairs influence their predisposition to pregnancy complicated by antiphospholipid syndrome (APS), preeclampsia (PE), intrauterine growth restriction (IUGR), and recurrent spontaneous abortion (RSA)
The most common HLA-G allele in the groups of women with antiphospholipid syndrome, preeclampsia, intrauterine growth restriction, and recurrent spontaneous abortion was the HLA-G 10101 allele found in 35.71%, 44.79%, 57.35%, and 52.59% of the women, respectively
Summary
During embryo implantation and pregnancy, the maternal immune system comes into close contact with fetal trophoblast cells. To avoid rejection of a semiallogeneic fetus, the mechanisms modulating the maternal immune system must be initiated [1]. The understanding of the molecular and biochemical characteristics of the human leukocyte antigen-G (HLA-G) and its products is important due to the diverse influence of this antigen’s polymorphisms on the course of the pregnancy. HLA-G was the first identified trophoblast HLA, Int. J. Res. Public Health 2019, 16, 982; doi:10.3390/ijerph16060982 www.mdpi.com/journal/ijerph
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