Abstract
The pathophysiology of diabetic neuropathic pain is due to primarily metabolic and vascular fac- tors. There is an increase in sorbitol and fructose, glycated end products, reactive oxygen species and activation of protein kinase C in the diabetic state. All these factors lead to direct damage to the nerves. Taking effective clinical management of neuropathic pain is based on a pharmacologi- cal treatment that has shown their limits and many side effects. The hypothesis of central sensiti- zation inhibited by Clerodendrum formicarum, an African pharmacopoeia plant used to treat headaches, arthritis, epilepsy and chronic pain could act on astrocytes and microglial cells. The objective of this work is to study the effect of Clerodendrum formicarum (100, 150 and 200 mg/kg body weight) on astrocytes and microglial cells in a model of diabetic neuropathic pain induced by alloxan monohydrate (150 mg/kg). We noted a suppression of mechanical allodynia and me- chanical hyperalgesia respectively by the Von Frey filaments test and the pressure test on the paw by the Clerodendrum formicarum extracts (ECF) at different doses from 2 h at the first injection of the ECF. After 5 days of treatment, we expressed by Western Blot bands of different proteins and by quantitative RT-PCR, we determined inhibition of the expression of GFAP, CD11b and isoforms 1 and 2 of cyclooxygenase. These results suggest that ECF inhibits the activation of astrocytes, mi- croglial cells and cyclooxygenase signaling pathway.
Highlights
Diabetic neuropathy is pathology of diabetic patient ignored because it is often silent
Statistical analysis allowed us to determine the ED50 for mechanical allodynia at 67.5 mg/kg and at 84 mg/kg for mechanical hyperalgesia. These results show that extracts of Clerodendrum formicarum (ECF) attenuates mechanical allodynia and mechanical hyperalgesia dose-dependent manner
We investigated the effect of ECF on COX1 and COX2 involved in neuropathy induced by the alloxan monohydrate (ALX)
Summary
Diabetic neuropathy is pathology of diabetic patient ignored because it is often silent This is one of the most common chronic complications of diabetes [1]. We will formulate a hypothesis about inhibition of central sensitization including glial cells in the dorsal horn of the spinal cord by the ethanol extracts of Clerodendrum formicarum (ECF). In this present work, our objective is to evaluate the effects of ECF in the treatment of painful diabetic neuropathy by this medication. Our objective is to evaluate the effects of ECF in the treatment of painful diabetic neuropathy by this medication To achieve this objective, we used two approaches: behavioral and biochemical
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