Abstract
Electrofocusing permits the resolution of crude fructose-bisphosphate aldolase (EC 4.1.2.13) from various tissues of rabbit and man into five forms corresponding to the different tetrameric hybrids of native and post-translationally modified chains. Subunits a and a′. The comparison of the distributions of these hybrids in red cells from rabbit and man of different ages confirms that only one of the two types of chains is synthesized. The rate of post-translational transformation of Subunits a and a′ is not the same in different tissues and moreover is slowing down during the life of the red cells. In all tissues, the distribution is nearly random.
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