Abstract

Phosphorylation, the reversible addition of phosphate groups to proteins, plays a pivotal role in regulating cellular processes, including signal transduction, cell cycle progression, and metabolism. Understanding the dynamics of phosphorylation events is crucial for unraveling complex signaling networks and identifying potential therapeutic targets in various diseases. Phosphorylation immunoassays have emerged as powerful tools for the detection and quantification of phosphorylated proteins, enabling researchers to gain insights into intricate cellular signaling pathways. This review provides an in-depth exploration of the recent advances, methodologies, and applications of phosphorylation immunoassays, highlighting their significance in advancing our understanding of cellular signaling.

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