Moisture content and mycorrhizal fungi in maternal environment influence performance and composition of Lallemantia species offspring

Abstract

The availability of soil water content and nutrition in the maternal plant environment plays pivotal roles in shaping the performance, physio-biochemical properties, and chemical composition of the produced seed. This study aimed to investigate the effects of water and arbuscular mycorrhizal fungi (AMF) of maternal plant environment on performance, physio-biochemical properties, and chemical compositions of Lallemantia species offspring. A split-factorial experiment was performed using a randomized complete block design (RCBD) with three replications. The main plot consisted of three drought stress (30 %, 60 % and 90 % of soil available water depletion). The subplots were the factorial combination of arbuscular mycorrhizal fungi (AMF− and AMF+) and Lallemantia species (L. iberica and L. royleana). The offspring of both Lallemantia species experienced a decrease in seed performance, superoxide dismutase, catalase, ascorbate peroxidase enzyme activities, proline, and chemical composition as well as a rise in hydrogen peroxide and lipid peroxidation due to the limited availability of water in the maternal plant environment. On the other hand, providing adequate nutrition in the maternal plant environment resulted in improved germination index, increased starch, and oil content, as well as higher levels of nitrogen and phosphorus in the offspring of both Lallemantia species. Compared to the offspring of L. royleana, the offspring of L. iberica had a higher number of achenes, seeds, seed weight, larger seed size, greater germination index, and higher levels of starch, oil, nitrogen, phosphorus, potassium, and calcium. In contrast, the offspring of L. royleana exhibited higher longevity, enhanced germination under osmotic and salinity stress, increased proline levels, and higher activities of antioxidant enzymes such as superoxide dismutase, catalase, and ascorbic peroxidase as well as sucrose and total soluble sugar. The study concludes that the best seed performance, antioxidant enzyme activities, and carbohydrate levels were observed in the offspring of both Lallemantia species produced under 60 % soil available water depletion with AMF inoculation in the maternal plant environment. These findings highlight the significant impact of the soil available water depletion and AMF inoculation on the seed performance, physio-biochemical properties, and chemical composition of the offspring, providing valuable insights for optimizing seed production and performance.