Abstract
A Gram-stain-negative, rod-shaped bacterium, strain 784B1_12E-CasoT, was isolated from a mixed manure sample used as input material of a German biogas plant. Phylogenetic identification based on the nearly full-length 16S rRNA gene sequence placed the strain within the family Flavobacteriaceae(Bacteroidetes) with highest sequence similarity to the type strain of Moheibacter sediminis (93.7 %) followed by Empedobacter brevis(90.7 %). Major cellular fatty acids of strain 784B1_12E-CasoT were iso-C15 : 0 and iso-C17 : 0 3-OH. The polyamine pattern contained predominantly sym-homospermidine and the quinone system consisted exclusively of menaquinone MK-6. Major polar lipids were phosphatidylethanolamine and an unidentified polar lipid only detectable after staining for total lipids. The DNA G+C content was 34.5 mol%. Based on phylogenetic, chemotaxonomic and physiological analyses, a novel species of the genus Moheibacter is proposed, Moheibacterstercoris sp. nov. (type strain 784B1_12E-CasoT=CIP 110830T=LMG 28502T).
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