Abstract
In this work, metal-organic frameworks (MOFs) FJU-21 was synthesized by solvothermal method. The crystal structure of FJU-21 was characterized by XRD and BET and it was applied to the catalytic hydrolysis of bovine serum albumin. The effects of reaction pH, temperature and reaction time on the catalytic activity of FJU-21 were studied. FJU-21 were found to possess an intrinsic enzyme mimicking activity similar to that found in trypsin. The Michaelis constant (Km) of the artificial protease (0.18 × 10-3 - 0.20 × 10-3 M-1) was about 15-fold lower than that free trypsin (2.7 × 10-3 M-1) and about 3-fold lower than that of soluble Cu(II) oxacyclen (0.54 × 10-3 M-1). The Kcat of FJU-21 is 102 times higher than that of soluble Cu(II) oxacyclen catalysts and, indicating a much higher affinity of BSA for FJU-21 surface. FJU-21 could be reused for eleven times without losing in its activity.
Highlights
The preparation and application of artificial mimic enzymes have become an increasingly important focus for research in recent years because natural enzymes have many problems such as a sensitivity to catalytic activity in the environment condition, lower stability, difficulty in purification and storage [1] [2]
Metal-organic frameworks (MOFs) FJU-21 was synthesized by solvothermal method
The crystal structure of FJU-21 was characterized by X-ray powder diffraction (XRD) and BET and it was applied to the catalytic hydrolysis of bovine serum albumin
Summary
The preparation and application of artificial mimic enzymes have become an increasingly important focus for research in recent years because natural enzymes have many problems such as a sensitivity to catalytic activity in the environment condition, lower stability, difficulty in purification and storage [1] [2]. Molybdenum peroxide amino complex, MoO(O2)2(a-leucine)(H2O) [14] was synthesized for photocatalytic hydrolysis of human pepsin These protein mimic enzymes have lower catalytic activity and poorer selectivity. MOFs have adjustable pore size, easy functionalization and extremely high specific surface area and porosity, making them suitable for catalysiss [15] [16], separation [17], gas storage [18], sensing, biomedical imaging and drug delivery and has attracted much attention in science and biological systems. These advantages of MOFs suggest that it to be a material suitable for enzyme mimics. We report that FJU-21 as protease mimetics to catalyze hydrolysis of bovine serum albumin (BSA)
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