Abstract
In this work, metal-organic frameworks (MOFs) Fe-MIL-101 was synthesized by hydrothermal method, and Fe/Fe-MIL-101 with different loadings was prepared. The crystal structure of the Fe/Fe-MIL-101 sample was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and specific surface area measurement (BET). Fe/Fe-MIL-101 was found to posses an intrinsic enzyme mimicking activity similar to that found in natural horse-radish peroxidase (HRP). The Michaelis constant (Km) of 5% Fe/Fe-MIL-101 with ABTS as the substrate is about 10-fold smaller than Fe-MIL-101 and about 3-fold smaller than HRP, and about 108 times less than that of CuO NPs (Km = 10.28 mM), indicating a much higher affinity for ABTS than HRP and most of the peroxidase mimetics.
Highlights
Natural enzymes have attracted much attention because of their high catalytic efficiency and strong response characteristics
The adsorption–desorption isotherms of 5% Fe/Fe-MIL-101 are of type I (Figure 1(b)), indicating the presence of a microporous network
Compared with Fe-MIL-101, the BET specific surface area of the experimentally synthesized 5% Fe/Fe-MIL-101 material decreased from 2737 m2/g to 2148 m2/g, and the pore volume decreased from 0.75 cm3·g−1 to 0.51 cm3·g−1, the average pore size decreased from 2.51 nm to 2.35 nm, the reduction of specific surface area, pore size and pore volume probably due to the blockage of material pores by the loaded iron
Summary
Natural enzymes have attracted much attention because of their high catalytic efficiency and strong response characteristics. Natural enzymes have many problems such as inactivation and denaturation, difficulty in purification, high cost and difficulty in storage and affected by environmental conditions including pH, temperature and inhibitors [1] [2] These defects largely limit the application of enzyme. A large number of peroxidase mimics, such as hemin [9] [10], hemeatin [11], hemoglobin [12], cyclodextrin [13], and porphyrin [14] [15], etc, have been used for H2O2 and ascorbic acid detection [13] [14] These peroxide mimic enzymes have lower catalytic activity and poorer selectivity than natural enzymes. It is necessary to work harder to design a mimetic enzyme with high catalytic activity
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