Modulatory response of chrysin supplementation in a experimental autoimmune encephalomyelitis model: Evaluation of microRNAs influence

Abstract

BackgroundMultiple sclerosis (MS) is a chronic autoimmune disease which causing demyelination, resulting in several damages to central nervous system. NonCoding microRNAs (miRNAs) are post transcriptional gene expression regulators that have been associated with MS-pathophysiology. In this context, chrysin is a bioactive compound that has several pharmacological, nutraceutical and neuroprotective effects. Thus, the aim of study was to investigate the effect of chrysin supplementation in alterations caused by experimental autoimmune encephalomyelitis (EAE) model in mice, analyzing histological parameters and changes in expression levels of MIR21, MIR155 and MIR326 (miRNAs-21, 155 and 326). MethodsFor induction of EAE was used myelin oligodendrocyte glycoprotein (35–55) peptide in C57BL/6 mice. EAE model caused increase of inflammatory infiltrate in SC and upregulated expression levels of miRNAs analyzed. ResultsSupplementation with chrysin was able to decrease of miRNAs-21 and 155 expression levels altered in EAE. Chrysin supplementation did not modulate miRNA-326 expression levels. ConclusionsThe findings suggest that chrysin attenuated inflammatory process presented in histological analysis, probably due to its indirect modulatory effect on miRNAs-21 and 155. Therefore, chrysin supplementation may represent an advance for MS therapeutic, however, further studies are needed to understand the role of miRNAs-21 and 155 in your modulatory response.