Modulatory effects of some natural products on hepatotoxicity induced by combination of sodium valproate and paracetamol in rats.

Abstract

Possible hepatoprotective effect of Curcuma longa and/or Nigella sativa against hepatotoxicity induced by coadministration of sodium valproate (SV) and paracetamol was studied. Rats were divided into 10 groups, control groups 1, 2, 3, and 4 received vehicles, C. longa (200mg/kg, p.o.), N. sativa (250mg/kg, p.o.), or both herbs for 21days, respectively. Toxicity groups 5, 6, and 7 received SV (300mg/kg, i.p.), paracetamol (1000mg/kg, p.o.) for the last 4days or both for 21days, respectively. Protection groups 8, 9, and 10 received C. longa, N. sativa, or both, respectively, 1h before the administration of both the drugs for 21days. SV and/or paracetamol significantly increased aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin, relative liver/body weight ratio, malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α), and caspase-3 (Casp-3) while significantly decreased albumin, total protein, glutathione (GSH) reduced, GSH peroxidase, and superoxide dismutase (SOD). Preadministration of C. longa and/or N. sativa caused protective effect against the hepatotoxicity induced by both drugs.