Modulatory effects of methanolic fruit fraction of Pedalium murex on sulphasalazine-induced male reproductive disruption.

Abstract

Pedalium murex is widely practiced in Ayurveda for the treatment of sexual disorders, but their detailed scientific evaluations are still unexplored. Therefore, the present study was conducted to assess the effect of methanolic fruit fraction of P.murex (MfPm) against sulphasalazine (SSZ) induced male reproductive disruption. MfPm and Clomiphene citrate were orally administered to SSZ (100mg/kg b.wt) induced infertile rats at the dose of 50 and 10mg/kg b.wt, respectively, for 60days. MfPm treatment promoted a significant (p<0.01) improvement in fertility (~70%), sperm motility (21%), and sperm density (11.20% and 12.30%). MfPm administration restored the serum luteinizing hormone, follicle-stimulating hormone, and testosterone levels back to their normal range in a significant (p<0.01) manner and also significantly (p<0.01) altered the level of biochemical parameters in treated rats. Furthermore, histological examination showed an improvement in spermatogenesis, as well as regeneration in the testicular architecture observed with increased germinal and interstitial cell count in response to MfPm treated rats. In conclusion, the results suggest that MfPm showed a significant modulatory effect against SSZ induced male reproductive disruption via possible mode of action such as spermatogenic and androgenic nature, therefore, justifying the traditional use of this plant in the treatment of reproductive disruption.