Modulatory Effects of Acute Aerobic Moderate Exercise on Purinergic Enzymes in Sedentary and Physically Active Males

Abstract

Exercise promotes biochemistry and physiological adaptations, leading to health benefits. Purinergic signaling is a well‐known system of cellular communication, based in extracellular nucleotides and nucleosides that modulate inflammatory, coagulatory and vascular activities. However, data about the effects of exercise in purinergic signaling remain limited. Thus, the main goal of present work was to evaluate the influence of acute aerobic moderate exercise on blood plasma nucleotidases in eutrophic sedentary (EUSED), overweight sedentary (OWSED) and physically active (ACT) male individuals. This study was approved by local Ethics Committee under protocol 2.452.427, being in agreement with the declaration of Helsinki. Twenty‐four healthy male individuals were divided into 3 groups: EUSED (N=8, BMI=23.51±2.01 Kg/m2, 26.38±2.97 years), OWSED (N=8, BMI=27±76 Kg/m2, 25.75±2.92 years) and ACT (N=8, BMI=23.96±1.35 Kg/m2, 23.13±3.18 years). Subjects were evaluated to collect clinical data and performed a cardiopulmonary exercise test to maximal oxygen uptake (VO2MAX) determination. Seven days after evaluation, subjects realized 30 minutes of exercise on treadmill with 70% VO2MAX. Blood samples were taken pre‐, post‐exercise and post‐1h of exercise. NTPDases and 5′‐nucleotidase activities were quantified by release of inorganic phosphate from ATP, ADP and AMP hydrolysis. NPP activity was assessed using p‐Nph‐5′‐TMP as substrate. Adenine nucleotides and nucleosides were quantified by HPLC. Data were analyzed using two‐way ANOVA repeated measures. ATP (EUSED: p=0.001; OWSED: p=0.015; ACT: p=0.002), ADP (EUSED: p=0.001; OWSED: p=0.009; ACT: p=0.001), AMP (EUSED: p=0.001; OWSED: p=0.001; ACT: p=0.001) and p‐Nph‐5′‐TMP (EUSED: p=0.001; OWSED: p=0.001; ACT: p=0.001) hydrolysis increased from pre‐ to post‐exercise among the groups. Post‐1h after exercise, ATP hydrolysis remained increased in ACT group (p=0.019), ADP in OWSED (p=0.03) and AMP in OWSED (p=0.001) and ACT (p=0.03). ATP (EUSED: p<0.001; OWSED: p<0.001; ACT: p<0.001) and ADP (EUSED: p<0.001; OWSED: p<0.001; ACT: p<0.001) plasma levels decreased from pre‐ to post‐exercise and remained decreased post‐1h after exercise among the groups. Adenosine (EUSED: p<0.001; OWSED: p=0.011; ACT: p=0.01), inosine (EUSED: p<0.001; OWSED: p<0.001; ACT: p<0.001) and uric acid (EUSED: p<0.001; OWSED: p=0.001; ACT: p=0.021) plasma levels increased from pre‐ to post‐exercise. Adenosine levels remained increased post‐1h exercise in all groups. There was no significant differences among the groups. Our results indicate that acute aerobic moderate exercise transiently increases NTPDases, 5′‐nucleotidase and NPP activities in plasma, decreasing levels of pro‐inflammatory and pro‐thrombotic molecules as ATP and ADP, increasing levels of cytoprotective molecules as adenosine and inosine in sedentary, overweight and physically active subjects.Support or Funding InformationNacional Council of Scientific and Technological Development (CNPq, Brazil) and Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil) supported this work.