Modulatory effect of advanced oxidation protein products on biological function of human first trimester trophoblast cells

Abstract

Objective To evaluate the effects of advanced oxidation protein products (AOPP) on the biological function of first-trimester trophoblast cell line (HTR8/SVneo). Methods HTR8/SVneo cells were cultured in vitro. Mouse serum albumin (MAS) was oxidized by hypochloric acid (HClO) to prepare AOPP-MAS in vitro. Based on concentration of AOPP-MAS used, the HTR8/SVneo cells were classified into a 50 μg/mL group, 100 μg/mL group, and 200 μg/ml group. Methyl thiazolyl tetrazolium (MTT) assay was used to assess the cell proliferation, which was showed by the mean absorbance (A) value. Transwell assay was used to measure cell invasion, which was showed by the number of cells passing the filter membrane. β-HCG secretion was used to assess the cell differentiation. The apoptosis of trophoblast cells was detected by Hoechest33258 staining. One-way ANOVA was used to compare the proliferation, invasive ability, β-HCG, and apoptosis of trophoblast cells treated with different concentrations of AOPP. Results Compared with the control group, the 50 μg/ml AOPP group had no significant change in the proliferation, invasion, differentiation, or apoptosis of HTR8SVneo cells (P>0.05). However, when AOPP concentration was 100 μg/ml or 200 μg/ml, AOPP significantly inhibited the proliferation, invasion, and differentiation of trophoblast cells, and increased the apoptosis of trophoblast cells (P<0.05). Conclusion AOPP could modulate trophoblast cell proliferation, invasion, and differentiation and induce apoptosis of trophoblast cells. Down-regulation of plasma AOPP levels may help to improve the biological function of trophoblast cells. Key words: Advanced oxidation protein products; Trophoblast cells; Proliferation; Invasion; Differentiation