Modulation Possibilities of the Tissue Wettability, Marker of the Physical Modifications Produced by Senescence versus Photo Senescence at Tegument Level

Laurentiu Badescu,Bogdan-Mihail Diaconescu,Carmen Elena Cotrutz,Raoul Lupusoru,Manuela Ciocoiu,Magda Badescu

doi:10.4236/jbise.2014.713102

Laurentiu Badescu, Bogdan-Mihail Diaconescu + Show 4 more

Open Access

https://doi.org/10.4236/jbise.2014.713102

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Abstract

We used an experimental model involving white Westar rats (young and old) who were subjected to precise doses of ultraviolet radiation (UV). The experiment aims to examine the influence of epidermis potential protection factors in photo senescence versus senescence. We have studied the in vivo effect of some natural polyphones extracted from black grapes seeds and zinc aspartate and in vitro effect of AED (deuterium depleted water). The substances were administered by intragastric gavages, at two days intervals for two weeks prior to UV irradiation and during the entire period of the experiment (28 days). In the end, we determined the degree of hydrophilicity of the skin, in vitro, by measuring the contact angle value, which was inversely proportional to the hydrophilicity of the tissue (the angle formed at the contact between a liquid and a solid surface). Using this method we found a large variability depending on the hydrophilicity of the epidermis (abdomen and back), the age of the animal and UV/non UV irradiation. The maintenance of the epidermis fragments in AED (for 6 hours) increased significantly the tissue wet ability degree both in the young rats subject to UV and in the senescent ones subject to UV (photo senescence).

Highlights

Chronic exposure to sunlight can cause premature aging of the skin and in some cases can promote tumor growth
By comparing the average values of the contact angle with the average values of the catalase or of the superoxiddismutase [11] in the groups of untreated control group of rats (Table 2), we can draw the following conclusions:—there are strong statistic differences both in the young rats and in the senescent ones;—the most significant differences are met for the catalase vs contact angle in the young rats, and in the senescent ones
By comparing the average values of the contact angle with the average values of the catalaseor of the superoxiddismutase, in the groups of untreated rats subject toultraviolet radiations (Table 3), we can draw the following conclusions:—there are strong statistical differences both in the young rats and in the senescent ones;— the most significant differences are met for the catalase vs contact angle in the senescent rats, and in the young ones (Table 4, Figure 6)

Summary

Introduction

Chronic exposure to sunlight can cause premature aging of the skin and in some cases can promote tumor growth. Aging caused by ultraviolet radiation (UV) exposure can manifest into two ways: immediate (short term) tanning with possible sunburns and late changes such as skin thickening, decreased skin elasticity and firmness loss [1]. The main body protection against solar radiation is the presence of melanin in melanocytes. Changes detected on skin biopsy revealed complex alterations on keratinocyte differentiation and kinetic and structural components of the skin in the context of photodamage [2] overlapped to the physiological senescence. The extracellular matrix, the main location of chemicals exchange loses hyaluronic acid with age and becomes less permeable, less hydrated and less fluid [3]

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