Abstract
The ability of different phosphorylation modulators to modify the opsonized zymosan (OZ)- and phorbol myristate acetate (PMA)-stimulated respiratory burst activity of rock bream ( Oplegnathus fasciatus) head-kidney phagocytes was investigated. OZ-stimulated respiratory burst was significantly inhibited by 5 nM of staurosporine, a protein kinase C (PKC) inhibitor, and completely inhibited by 10 nM of staurosporine, whereas PMA-stimulated respiratory burst was completely inhibited by both 5 and 10 nM of staurosporine. Although genistein, a protein tyrosine kinase (PTK) inhibitor, inhibited significantly both OZ- and PMA-stimulated respiratory burst, OZ-stimulated respiratory burst was inhibited much more selectively than PMA-stimulated one. OZ- and PMA-stimulated respiratory burst were increased significantly by sodium orthovanadate, a protein tyrosine phosphatase inhibitor, and the increased rate of OZ-stimulated respiratory burst was markedly higher than that of PMA-stimulated one. These results suggest that OZ-stimulated respiratory burst is more dependent on PTK while the PMA-stimulated one is more dependent on PKC, and the balance between tyrosine kinase and phosphotyrosine phosphatase activity is important in determining the intensity of respiratory burst in phagocytes.
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