Abstract
The possibility that TSH is required for maintenance of thyroglobulin (TG) messenger RNA (mRNA) levels in the thyroid was tested by producing TSH deficiency in Wistar Furth rats. Concentrations of TG mRNA in the thyroid cytoplasmic RNAs of treated and control rats were compared by the relative rates of hybrid formation with TG complementary DNA. The products of the concentration of the TG mRNA times the amount of RNA per thyroid DNA were used to compare the amounts of TG mRNA on a cellular basis in each test condition. The results showed that rats made TSH-deficient either by suppression with high doses of T4 (100–150 μg daily–100 g BW for 7–10 days) or by hypophysectomy underwent a decrease in amount of TG mRNA in excess of the diminution of other RNA classes. The RNA content of the cytoplasm decreased by 32–38% in four groups of T4-treated animals, whereas the cytoplasmic TG mRNA content decreased by 62–70%. In four groups of hypophysectomized animals, fractional changes were more variable, with the cyt...
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