Abstract
Objective To investigate the roles of micro RNA(miRNA)-221 and miRNA-222 on the proliferation and migration of prostate cancer cell PC-3 and the influence on silent information regulator 1(SIRT1). Methods The groups were arranged as cells without treatment(control group),cells transfected with pEX-5 empty plasmid(pEX-5 group), cells transfected with miRNA-221 inhibitor(miRNA-221 inhibition group) and cells transfected with miRNA-222 inhibitor(miRNA-222 inhibition group). The influence of miRNA-221 and miRNA-222 on prostate cancer cell proliferation was determined by CCK-8,and the migration ability was determined by wound healing test. The protein and mRNA levels of SIRT1 were determined by Western blotting and fluorescence quantitation polymerase chain reaction(PCR),respectively. The prediction analysis was performed by miRNAanda target. Results After the expression inhibition of miRNA-221 or miRNA-222 in cell PC-3,the cell activities were determined for 7 d. The activities(A450 mm) in miRNA-221 and miRNA-222 inhibition groups were down-regulated about 1. 5-2. 0 times compared with that in pEX-5 group,and there were significant differences between the 2 groups from the 3rd d(t = 6. 7,P 0. 01; t = 5. 3,P 0. 01). The wound healing test showed that the migration abilities of miRNA-221 inhibition group and miRNA-222 inhibition group decreased than that of pEX-5 group. Moreover,the expression levels of SIRT1 protein in miRNA-221 inhibition group(0. 26 ± 0. 021) and miRNA-222 inhibition group(0. 21 ± 0. 005 8) were up-regulated compared with that in pEX-5 group(0. 14 ± 0. 017),and there were statistical significances(t = 6. 3 and 6. 4,P 0. 05). The SIRT1 mRNA expression levels had no statistical significance among the 3 groups(P 0. 05). Conclusions The miRNA-221 and miRNA-222 promote the proliferation and migration of prostate cancer cell PC-3 and have the influence on SIRT1 expression in protein level.
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