Modulation of the Effect of Doxorubicin and Medroxyprogesterone Acetate on Cytokine and Oxidant Activity of Human Leukocytes by Hanks' Cold Plasma-Treated Solution

Abstract

Cold plasma and plasma-treated solutions (PTS) have recently become widely adopted for the treatment of cancer and inflammatory diseases, particularly in concert with other medications. These complexes affect cytokine and oxidative activity of leukocytes, which are important in the pathogenesis of a variety of diseases. In this work, we study the variations in the immune response of healthy leukocytes when doxorubicin, medroxyprogesterone acetate, and PTS affect them separately or in combination. Employing the "CAPKO-JET" plasma source, we achieve an increase in the anti-tumor immune response from the combination of PTS with doxorubicin and MPA at treatment times shorter than 2.5 min. In contrast, the anti-inflammatory immune response is amplified at treatment times of 7.5 min or more. Applying PTS without the above medications upon the completion of the first phase of the inflammation therapy at the injury site is suggested to accelerate healing.