Modulation of the Antibiotic Activity by Extracts fromAmburana cearensisA. C. Smith andAnadenanthera macrocarpa(Benth.) Brenan

Fernando G Figueredo,José G M Costa,Cícero M G Torres,Henrique D M Coutinho,Emerson O Ferreira,Cássio R Medeiros,Júlio C F Silva,Daniel L Lucetti,Irwin R A Menezes,Edinardo F F Matias,Elaine C P Lucetti,Gardênia M M Oliveira,Francisco A V Santos,Aracélio V Colares,Bruno F F Lucena,João Marcos F L Silva,Patrícia R L Figueiredo,Marta R Kerntopf

doi:10.1155/2013/640682

Abstract

The aim of this study was to verify the possible interactions between ethanol extracts of Amburana cearensis A. C. Smith and Anadenanthera macrocarpa (Benth.) Brenan, combined with six antimicrobial drugs against multiresistant strains of Staphylococcus aureus and Escherichia coli isolated from humans. The antibacterial activity of the extracts was determined using the minimum inhibitory concentration (MIC). The microdilution assay was performed to verify the interactions between the natural products and the antibiotics using a subinhibitory concentration. The activity of amikacin associated with the extract of Anadenanthera macrocarpa against EC 27 was enhanced, demonstrating an MIC reduction from 128 to 4 μg/mL. Among the β-lactams, no potentiation on its activity was observed, with exception to the antagonism of the natural products with ampicillin against S. aureus 358.

Highlights

E research for new antibacterial substances becomes necessary due to increase of the antibiotic resistance of clinically important pathogens [1]
Smith and Anadenanthera macrocarpa (Benth.) Brenan, combined with six antimicrobial drugs against multiresistant strains of Staphylococcus aureus and Escherichia coli isolated from humans. e antibacterial activity of the extracts was determined using the minimum inhibitory concentration (MIC). e microdilution assay was performed to verify the interactions between the natural products and the antibiotics using a subinhibitory concentration. e activity of amikacin associated with the extract of Anadenanthera macrocarpa against EC 27 was enhanced, demonstrating an MIC reduction from 128 to 4 μμg/mL
Leaves of Amburana cearensis and Anadenanthera macrocarpa were collected at Penaforte, Ceara, Brazil. e plant material was identi ed and a voucher specimen was placed in the respective herbal collections (Table 2)

Summary

Introduction

E research for new antibacterial substances becomes necessary due to increase of the antibiotic resistance of clinically important pathogens [1]. Due to this fact, substances derived from plants could be attractive alternatives [2, 3]. Known as Cumaru , it has been explored for use in ne furniture making, sculpture and carpentry, being listed as a threatened species [11] Due to their medicinal properties, the bark and seeds are used to produce popular drugs for the treatment of cough, asthma, bronchitis, and pertussis. Cearensis and A. macrocarpa to determine the antibacterial activity and the modifying antibiotic activity of aminoglycosides and beta-lactams against the Escherichia coli and Staphylococcus aureus

Material and Methods

Results and Discussion

Conclusion