Abstract
目的 研究免疫调节剂咪喹莫特对卵蛋白(OVA)致敏大鼠支气管旁淋巴结(PBLN)细胞培养体系中T辅助淋巴细胞(Th)亚群产生细胞因子的影响,并探讨其作用机制.方法建立PBLN细胞培养体系,并按不同浓度分为A～F组进行干预.在培养0、3、6、12、24、48 h后,各组分别取5孔细胞培养液,用酶联免疫吸附法(ELISA)检测各细胞因子的蛋白质水平,用逆转录聚合酶链反应(RT-PCR)检测细胞沉淀中白细胞介素(IL)-4和干扰素(IFN)-γ等细胞因子的mRNA表达.结果在A组的各时间点PBLN细胞培养液中,仅可检测到少量IFN-γ.随着培养时间的延长,咪喹莫特浓度为1、10 μg/ml的E和F组与B组相比,IL-4及相关mRNA水平增加缓慢, IFN-γ水平增长迅速(均P 0.05).结论咪喹莫特对致敏大鼠PBLN细胞培养体系中Th亚群产生细胞因子的最佳作用发生在12 h时,提示咪喹莫特可能在支气管哮喘等特异性疾病的迟发炎症反应阶段发挥重要作用。
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