Modulation of radiation- and cadmium-induced biochemical changes in the jejunum of Swiss albino mice by polybion

Abstract

This study aims to evaluate the protective effect of polybion against radiation- and cadmium-induced biochemical changes in the jejunum of Swiss albino mice. The animals were exposed with 2.0 or 4.0 Gy of gamma rays with or without cadmium chloride treatment. In the drug treated groups, the polybion was given seven days prior to irradiation or cadmium chloride treatment. The animals from all the experimental groups were sacrificed by cervical dislocation at posttreatment intervals of 1, 2, 4, 7, 14 and 28 days. The values of total proteins, glycogen, cholesterol, DNA, RNA, acid phosphatase and alkaline phosphatase activity were estimated. The values of total proteins, glycogen, cholesterol, DNA and RNA decreased up to day 14 in non-drug-treated groups and day 7 in the polybion-treated groups, and thereafter increased. The values of acid phosphatase and alkaline phosphatase activity increased up to day 14 in non-drug-treated groups, and day 7 in the drug-treated groups, then declined in all groups. Severe changes were observed after combined exposure, showing a synergistic effect. An early recovery and less severe changes in the drug-treated groups indicated protection by polybion. Thus, it appears that polybion is potent enough to check cadmium- and radiation-induced biochemical alterations in the jejunum of Swiss albino mice.