Abstract
The interactions between bovine mononuclear cells and Haemophilus somnusare known to be complex. To study this interaction, a flow cytometric assay was developed to assess the effect of H. somnuson phagocytosis of killed opsonized Staphylococcus aureusby bovine alveolar macrophages and blood monocytes. Using this in vitrosystem, it was found that log phase H. somnussignificantly inhibited the phagocytosis of killed opsonized S. aureusby bovine alveolar macrophages obtained both from healthy calves and from cattle experimentally infected with H. somnus. However, killed log-phase H. somnus, in vitropassaged and stationary phase H. somnushad no effect on the phagocytic activity of these cells. In contrast to bovine alveolar macrophages, blood monocytes showed a significant increase in their phagocytic activity following in vitroexposure to either log or stationary phase H. somnus. Using a lypophilic, non-toxic fluorophore PKH2 to label live H. somnus, it was possible to simultaneously measure the uptake of both S. aureusand H. somnus. Stationary and log phase H. somnuswere taken up by macrophages equally well, even though phagocytosis of S. aureuswas inhibited by only log phase H. somnus. These results demonstrate the ability of H. somnusto modulate bovine mononuclear phagocytic function which might contribute towards the pathogenesis of bovine hemophilosis.
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